Product Name | ATP ELISA kit (General species) |
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Description | This assay has high sensitivity and excellent specificity for detection of Adenosine Triphosphate (ATP). No significant cross-reactivity or interference between Adenosine Triphosphate (ATP) and analogues was observed. |
Synonyms | Adenosine-5'-Triphosphate |
Method | Competitive |
Detection Range | 12.35-1,000ng/mL |
Sensitivity | 5.25ng/mL |
Target | ATP |
Specificity | This assay has high sensitivity and excellent specificity for detection of Adenosine Triphosphate (ATP). No significant cross-reactivity or interference between Adenosine Triphosphate (ATP) and analogues was observed. |
Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately. |
Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
Notes | Colormetric |
References | Effects of Aerobic Training, with or without Zizyphus Jujuba Water Extraction, on Fundus Nesfatin-1, ATP, HDL-C, and LDL-C Concentrations in Female Rats;Neuroprotective effects of vildagliptin in rat rotenone Parkinson's disease model: role of RAGE‐NFκB and Nrf2‐antioxidant signaling pathways;Metabolic Features of Heart Failure with Different Etiology ;Rifampicin-induced injury in L02 cells is alleviated by 4-PBA via inhibition of the PERK-ATF4-CHOP pathway.;Rifampicin-induced injury in HepG2 cells is alleviated by TUDCA via increasing bile acid transporters expression and enhancing the Nrf2-mediated adaptive response.;The Antioxidative Action of ZTP by Increasing Nrf2/ARE Signal Pathway;Antihypoxic and anti-ischemic properties of the North Caucasus flora plant extracts;Effects of nanoparticles on Neuroinflammation in a Mouse Model of Asthma;Novel complex of cosmetic ingredients with promising action in preventing hair loss and follicular aging through mechanism involving enrichment of WNT/signaling …;Ethylmethylhydroxypyridine succinate, acetylcysteine and choline alphoscerate improve mitochondrial function under condition of cerebral ischemia in rat;Prostaglandin E receptor subtype 4 protects against diabetic cardiomyopathy by modulating cardiac fatty acid metabolism via FOXO1/CD36 signalling;Serum Metabolomic Analysis of Coronary Heart Disease Patients with Stable Angina Pectoris Subtyped by Traditional Chinese Medicine Diagnostics Reveals ¡;Estrogen inhibits bladder overactivity in rats with cyclophosphamide‐induced cystitis via downregulating the expression of P2X3 receptors in bladder epithelium cells;A Famous Chinese Medicine Formula: Yinhuo Decoction Antagonizes the Damage of Corticosterone to PC12 Cells and Improves Depression by Regulating …; |
Supplier | Cloud Clone |
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