| Product Name | ATP2A2 ELISA kit (Human) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2). No significant cross-reactivity or interference between ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) and analogues was observed. |
| Synonyms | ATP2B; DAR; SERCA2; Calcium pump 2; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle |
| Method | Sandwich |
| Detection Range | 0.312-20ng/mL |
| Sensitivity | 0.115ng/mL |
| Target | ATP2A2 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2). No significant cross-reactivity or interference between ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2 (ATP2A2) and analogues was observed. |
| Reactivity | Human |
| Sample Types | Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P16615 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | SERCA2a: A potential non-invasive biomarker of cardiac allograft rejection.;Value of SERCA2a as a Biomarker for the Identification of Patients With Advanced Heart Failure Requiring Circulatory Support;Value of SERCA2a as a Biomarker for the Identification of Patients with Heart Failure Requiring Circulatory Support; |
| Supplier | Cloud Clone |
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