Bax ELISA kit (Rat)

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SEB343Ra
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Product Name Bax ELISA kit (Rat)
Description This assay has high sensitivity and excellent specificity for detection of Bcl2 Associated X Protein (Bax). No significant cross-reactivity or interference between Bcl2 Associated X Protein (Bax) and analogues was observed.
Synonyms BCL2L4; Bax Zeta; Apoptosis regulator BAX; Bcl-2-like protein 4
Method Sandwich
Detection Range 0.312-20ng/mL
Sensitivity 0.125ng/mL
Target Bax
Specificity This assay has high sensitivity and excellent specificity for detection of Bcl2 Associated X Protein (Bax). No significant cross-reactivity or interference between Bcl2 Associated X Protein (Bax) and analogues was observed.
Reactivity Rat
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Neurotoxic effect of lead on rats: Relationship to Apoptosis;Effects of hepatocyte growth factor in myocarditis rats induced by immunization with porcine cardiac myosin;Cu (II) (PICOLINYL-L-TRYPTOPHAN)2 AS AN EFFECIENT MULTIFUNCTIONAL RADIOPROTECTOR;Mechanistic insights into the effects of quercetin and/or GLP-1 analogue liraglutide on high-fat diet/streptozotocin-induced type 2 diabetes in rats;A polysaccharide (PNPA) from Pleurotus nebrodensis ameliorates hepatic ischemic/reperfusion (I/R) injury in rats;Potentiation of Apoptosis in Rat Striatum Exposed to Endosulfan and the Role of Quercetin;Cardioprotective effect of the xanthones from Gentianella acuta against myocardial ischemia/reperfusion injury in isolated rat heart;Taxifolin protects rat against myocardial ischemia/reperfusion injury by modulating the mitochondrial apoptosis pathway;LCZ696 (sacubitril/valsartan) protects against cyclophosphamide-induced testicular toxicity in rats: Role of neprilysin inhibition and lncRNA TUG1 in ameliorating …;Potentiation of the apoptotic signaling pathway in both the striatum and hippocampus and neurobehavioral impairment in rats exposed chronically to a low− dose of …;Alogliptin attenuates cyclophosphamide-induced nephrotoxicity: a novel therapeutic approach through modulating MAP3K/JNK/SMAD3 signaling cascade;Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats;Potential effect of adrenomedullin on metabolic and endocrinal dysfunctions in the experimentally induced polycystic ovary: Targeting implication of endoplasmic ¡­;Galangin mitigates oxidative stress, inflammation, and apoptosis in a rat model of methotrexate hepatotoxicity;Selenium nanoparticles overcomes sorafenib resistance in thioacetamide induced hepatocellular carcinoma in rats by modulation of mTOR, NF-κB pathways and …;Piracetam attenuates cyclophosphamide-induced hepatotoxicity in rats: Amelioration of necroptosis, pyroptosis and caspase-dependent apoptosis;
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