| Product Name | BCAS2, 1-225aa, Human |
|---|---|
| Description | BCAS2, also known as pre-mRNA-splicing factor SPF27, is a ubiquitously expressed nuclear protein that was originally identified as being overexpressed in various breast cancer cell lines. It is now known to be a component of the spliceosome, participating in the removal of introns from mRNA precursors. BCAS2 specifically interacts (in a ligand-independent manner) with TRbeta (thyroid hormone receptor beta), ERalpha (estrogen receptor alpha), ERbeta, PR (progesterone receptor) and PPARgamma (peroxisome proliferator-activated receptor gamma). This protein functions as an ER co-activator and is capable of enhancing ER-mediated transcription. This suggests that BCAS2 is involved in the development of breast cancer. Recombinant human BCAS2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Synonyms | pre-mRNA-splicing factor SPF27, DAM1, Snt307, SPF27 |
| Host | E. coli |
| Molecular Weight | 23.8kDa (207aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher) |
| Amino Acid Sequence | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAGT GLVAGEVVVD ALPYFDQGYE APGVREAAAA LVEEETRRYR PTKNYLSYLT APDYSAFETD IMRNEFERLA ARQPIELLSM KRYELPAPSS GQKNDITAWQ ECVNNSMAQL EHQAVRIENL ELMSQHGCNA WKVYNENLVH MIEHAQKELQ KLRKHIQDLN WQRKNMQLTA GSKLREMESN WVSLVSKNYE IERTIVQLEN EIYQIKQQHG EANKENIRQD F |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 100mM NaCl, 1mM PMSF |
| Concentration | 0.5mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Qi C., et al. (2005) Biochem Biophys Res Commun. 328(2):393-8. Kuo PC., et al. (2009) Cancer Res. 69(23):8877-85. |
| Background | BCAS2, also known as pre-mRNA-splicing factor SPF27, is a ubiquitously expressed nuclear protein that was originally identified as being overexpressed in various breast cancer cell lines. It is now known to be a component of the spliceosome, participating in the removal of introns from mRNA precursors. BCAS2 specifically interacts (in a ligand-independent manner) with TRbeta (thyroid hormone receptor beta), ERalpha (estrogen receptor alpha), ERbeta, PR (progesterone receptor) and PPARgamma (peroxisome proliferator-activated receptor gamma). This protein functions as an ER co-activator and is capable of enhancing ER-mediated transcription. This suggests that BCAS2 is involved in the development of breast cancer. Recombinant human BCAS2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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