| Product Name | BDNF ELISA kit (Rat) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Brain Derived Neurotrophic Factor (BDNF). No significant cross-reactivity or interference between Brain Derived Neurotrophic Factor (BDNF) and analogues was observed. |
| Synonyms | Neurotrophin; Abrineurin |
| Method | Sandwich |
| Detection Range | 31.2-2,000pg/mL |
| Sensitivity | 2.9pg/mL |
| Target | BDNF |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Brain Derived Neurotrophic Factor (BDNF). No significant cross-reactivity or interference between Brain Derived Neurotrophic Factor (BDNF) and analogues was observed. |
| Reactivity | Rat |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P23363 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young rats;Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons.;N-3 PUFA diet enrichment prevents amyloid beta-induced depressive-like phenotype;Amelioration by nitric oxide (NO) mimetics on neurobehavioral and biochemical changes in experimental model of Alzheimer's disease in rats;Chronic harmine treatment has a delayed effect on mobility in control and socially defeated rats;Involvement of neurotrophic signaling in doxorubicin‑induced cardiotoxicity;Hyperlipidemia Down‐Regulate Brain Antioxidant Defense Enzymes and Neurotrophins in Rats: Assessment of the Modulatory Potential of EPA+ DHA and …;Short-but no long-term effect of social stress in rodent depressive-like behavior is not affected by chronic treatment with Harmine;Overektomize dişi sıçanlarda beyin oksidatif stres, bdnf ve inflamatuar cevap düzeyleri ile nörodavranışsal değişiklikler üzerine anjiyotensin II İnhibisyonunun etkisi;Lithium augmentation of ketamine increases insulin signaling and antidepressant-like active stress coping in a rodent model of treatment-resistant depression;Adrenomedullin increases cAMP accumulation and BDNF expression in rat DRG and spinal motor neurons;Endogenous Deficiency of Brain-Derived Neurotrophic Factor Induces the Downregulation of Tryptophan Hydroxylase-2 Expression in Raphe Nuclei of Rapid …; |
| Supplier | Cloud Clone |
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