| Product Name | BECN1, 1-450aa, Human |
|---|---|
| Description | BECN1, also as known as Beclin-1 and autophagy-related gene (Atg) 6, belongs to the beclin family. This protein plays a central role in autophagy. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagasome in autophagy. Also it required for the abcission step of cytokinesis. BECN1 plays a role in antiviral host defense. Recombinant human BECN1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. |
| Synonyms | beclin 1, ATG6, beclin1, VPS30 |
| Host | E. coli |
| Molecular Weight | 23.5 kDa (209aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MEGSKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELED VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ LELDDELKSV ENQMRYAQTQ LDKLKKTNVF NATFHIWHSG QFGTINNFRL GRLPSVPVEW NEINAAWGQT VLLLHALANK MGLKFQRYRL VPYGNHSYLE SLTDKSKELP LYCSGGLRFF WDNKFDHAMV AFLDCVQQFK EEVEKGETRF CLPYRMDVEK GKIEDTGGSG GSYSIKTQFN SEEQWTKALK FMLTNLKWGL AWVSSQFYNK |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 2M Urea, 20% glycerol, 0.2M NaCl |
| Concentration | 0.5 mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Sagona A.P., et al. (2010) Nat. Cell Biol. 12:362-371 Itakura E., et al. (2008) Mol. Biol. Cell 19:5360-5372 |
| Background | BECN1, also as known as Beclin-1 and autophagy-related gene (Atg) 6, belongs to the beclin family. This protein plays a central role in autophagy. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagasome in autophagy. Also it required for the abcission step of cytokinesis. BECN1 plays a role in antiviral host defense. Recombinant human BECN1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. |
| Supplier | ARP |
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