| Product Name | beta Amylase Activity Assay Kit |
|---|---|
| Description | This beta Amylase Activity Assay Kit is a quantitative assay kit for measuring beta Amylase activity in serum, plasma, saliva, animal and plant tissues. |
| Method | Quantitative |
| Detection Range | 0.0156-1 mg/ml |
| Sensitivity | 0.0078 mg/ml |
| Target | beta-Amylase |
| Applications | Functional Studies |
| Sample Types | Serum, plasma, saliva, animal tissues and plant tissues. |
| Reconstitution | This kit provides a convenient tool for sensitive detection of beta-amylase activity. The principle is that amylase catalyzes the hydrolysis of starch to form reducing sugars, and 3,5-dinitrosalicylic acid is reduced by reducing sugar to produce a brown-red substance with an absorption peak at 540 nm; the amylase activity is calculated by measuring the rate of increase in absorbance at 540 nm. Alpha-amylase is heat-resistant, but beta-amylase can be inactivated at 70°C. Therefore, only alpha-amylase can catalyze starch hydrolysis after the crude enzyme solution is passivated at 70°C. The total amylase activity is measured with the crude enzyme solution that is not heat-treated, and the beta-amylase activity can be obtained by subtracting the alpha-amylase form total amylase activity. |
| Storage | Shipped at 4°C. Store at 4°C in the dark. Do not use past expiration date! |
| Supplier | Antibodies.com |
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