Biotinylated human Fc gamma RIIIb / CD16b (NA2) protein

Category: Proteins
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Product Name Biotinylated human Fc gamma RIIIb / CD16b (NA2) protein
Description The sequence of the extracellular domain of human CD16b (Gly 17-Ser 200) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag. The full protein sequence can be downloaded from the product webpage.
Source Human
Conjugate Biotin
Molecular Weight The recombinant human CD16b (NA2) including tag consists of 223 amino acids and has a theoretical mass of 25289 Da.
Expression System Human embryonic kidney (HEK) 293 cells.
Form Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added.
Purity >95% monomer purity as determined by SDS-PAGE and SEC-HPLC.
Gene Id O75015
Storage Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial.
Notes Conjugation notes: Biotinylated. Biotin to protein ratio is confirmed as 0.7-1.0 by the HABA assay. Product has been site-specifically biotinylated using the AVI tag technology, where the lysine residue within the tag is enzymatically labeled with biotin. .. Allotype: NA2. Reconstitution: To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water.
Supplier Gamma Proteins

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