| Product Name | Biotinylated human Fc gamma RIIIb / CD16b (NA2) protein |
|---|---|
| Description | The sequence of the extracellular domain of human CD16b (Gly 17-Ser 200) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag. The full protein sequence can be downloaded from the product webpage. |
| Synonyms | CD16B, FCGR3B, FCGRIIIB, FCR3B, FCRIIIB, IGFR3B, IGFRIIIB |
| Source | Human |
| Conjugate | Biotin |
| Molecular Weight | The recombinant human CD16b (NA2) including tag consists of 223 amino acids and has a theoretical mass of 25289 Da. |
| Expression System | Human embryonic kidney (HEK) 293 cells. |
| Form | Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added. |
| Purity | >95% monomer purity as determined by SDS-PAGE and SEC-HPLC. |
| Gene Id | O75015 |
| Storage | Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommend storage at 4 °C for up to one week. For longer term storage of protein in solution we recommend aliquoting into smaller vials to avoid repeated freeze-thaw cycles and storage at -20 or -80 °C for up to 3 months. To avoid surface adsorption loss and inactivation we strongly recommend that the protein should not be aliquoted in less than 10 μg per vial. |
| Notes | Conjugation notes: Biotinylated. Biotin to protein ratio is confirmed as 0.7-1.0 by the HABA assay. Product has been site-specifically biotinylated using the AVI tag technology, where the lysine residue within the tag is enzymatically labeled with biotin. .. Allotype: NA2. Reconstitution: To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water. |
| Supplier | Gamma Proteins |
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