| Product Name | BLUeye Prestained Protein Ladder |
|---|---|
| Description | Pre-stained protein ladder. Covers the size range 10-245 kDa (Tris-glycine-SDS running buffer). Number of bands = 12. Green band at approximately 25 kDa. Red band at approximately 75 kDa. Designed for monitoring SDS-PAGE separation and WB transfer efficiency. Thaw at room temperature or 37-40°C for a few minutes prior to use to dissolve precipitated solids. Do not boil. Do not heat, dilute, or add reducing agent before loading. Mix thoroughly. |
| Applications | SDS-PAGE, WB |
| Working Dilutions | Load 5 µl/well (mini-gels) or 10 µl/well (large gels). For WB load 2.5 µl and 5 µl, respectively. |
| Form | Liquid |
| Diluent Buffer | 20 mM Tris-Phosphate pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea and 15% (v/v) Glycerol. |
| Concentration | 0.1-0.4 mg/ml of each protein |
| Storage | Shipped at 4°C. Store at 25°C for up to 2 weeks, 4°C for up to 3 months, and -20°C for long-term storage. |
| Supplier | Antibodies.com |
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