| Product Name | C3a ELISA kit (Human) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Complement Component 3a (C3a). No significant cross-reactivity or interference between Complement Component 3a (C3a) and analogues was observed. |
| Method | Sandwich |
| Detection Range | 0.78-50pg/mL |
| Sensitivity | 30pg/mL |
| Target | C3a |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Complement Component 3a (C3a). No significant cross-reactivity or interference between Complement Component 3a (C3a) and analogues was observed. |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P01024 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Immobilization of soluble complement receptor 1 on islets.;Elevated complement factor C5a in maternal and umbilical cord plasma in preeclampsia;Complement Split Products C3a/C5a and Receptors: Are They Regulated by Circulating Angiotensin II Type 1 Receptor Autoantibody in Severe Preeclampsia?;Acute and prolonged complement activation in the central nervous system during herpes simplex encephalitis;Interaction of gold and silver nanoparticles with human plasma: Analysis of protein corona reveals specific binding patterns.;Intrathecal complement activation by the classical pathway in tick-borne encephalitis;Hemocompatibility of biodegradable Zn-0.8 wt%(Cu, Mn, Li) alloys;Complement-mediated hypersensitivity reactions to an amphotericin B-containing lipid complex (Abelcet) in pediatric patients and anesthetized rats: Benefits of slow infusion;Increased ratios of complement factors C3a to C3 in aqueous humor and serum mark glaucoma progression;C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease; |
| Supplier | Cloud Clone |
PROTOCOLS:
SEA387Hu All Research Products are sold for laboratory RESEARCH USE ONLY and ARE NOT TO BE USED FOR HUMAN OR ANIMAL THERAPEUTIC OR DIAGNOSTIC APPLICATIONS. The information presented is believed to be accurate; however, said information and products are offered without warranty or guarantee since the ultimate conditions of use and the variability of the materials treated are beyond our control. Nothing disclosed herein is to be construed as a recommendation to use our products in violation of any patents. ARP American Research Products, Inc. does not submit its products for regulatory review by any government body or other organization, and we do not validate them for clinical, therapeutic or diagnostic use, or for safety and effectiveness. You are solely responsible for making sure that the way you use the products complies with applicable laws, regulations and governmental policies and for obtaining all necessary approvals, intellectual property rights, licenses and permissions that you may need related to your use. Under no circumstances shall ARP American Research Products, Inc. be liable for damages, whether consequential, compensatory, incidental or special, strict liability or negligence, breach of warranty or any other theory arising out of the use of the products available from ARP American Research Products, Inc. Nothing contained herein warrants that the use of the products will not infringe on the claims of any patents covering the product itself or the use thereof in combination with other products or in the operation of any process. ARP American Research Products, Inc. disclaims any and all representations or warranties of any kind whatsoever, express or implied, including without limitation any implied warranties of merchantability or fitness for a particular purpose, of non-infringement, or regarding results obtained through the use of any product, whether arising from a statute or otherwise in law or from a course of performance, dealing or usage of trade.
