| Product Name | CALR3, 20-384aa Human |
|---|---|
| Description | Calreticulin 3, also known as CALR3, is a member of the calreticulin (CRT) family. It is calcium-binding chaperones that are localized to the endoplasmic reticulum or sarcoplasmic reticulum in eukaryotes. CALR3 assists in the calreticulin/calnexin cycle, where it is involved in protein-folding, oligomeric assembly and quality control in the ER. With specific expression in testis, CALR3 has been implicated as a cancer-testis antigen due to its frequent expression in various cancers. Recombinant human CALR3 protein was expressed in E.coli. |
| Synonyms | Calreticulin 3, CRT2, FLJ25355, MGC26577 |
| Host | E. coli |
| Molecular Weight | 30.2kDa (272aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MTVYFQEEFL DGEHWRNRWL QSTNDSRFGH FRLSSGKFYG HKEKDKGLQT TQNGRFYAIS ARFKPFSNKG KTLVIQYTVK HEQKMDCGGG YIKVFPADID QKNLNGKSQY YIMFGPDICG FDIKKVHVIL HFKNKYHENK KLIRCKVDGF THLYTLILRP DLSYDVKIDG QSIESGSIEY DWNLTSLKKE TSPAESKDWE QTKDNKAQDW EKHFLDASTS KQSDWNGDLD GDWPAPMLQK PPYQDGLKPE GIHKDVWLHR KMKNTDYLTQ YDLSEFENIG AIGLELWQVR SGTIFDNFLI TDDEEYADNF GKATWGETKG PEREMDAIQA KEEMKKAREE EEEELLSGKI NRHEHYFNQF HRRNEL |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Persson S., et al. (2002) Gene. 297: 151-158. Michalak M., et al. (1998) Biochem Cell Biol. 76: 779-785. |
| Background | Calreticulin 3, also known as CALR3, is a member of the calreticulin (CRT) family. It is calcium-binding chaperones that are localized to the endoplasmic reticulum or sarcoplasmic reticulum in eukaryotes. CALR3 assists in the calreticulin/calnexin cycle, where it is involved in protein-folding, oligomeric assembly and quality control in the ER. With specific expression in testis, CALR3 has been implicated as a cancer-testis antigen due to its frequent expression in various cancers. Recombinant human CALR3 protein was expressed in E.coli. |
| Supplier | ARP |
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