| Product Name | cAMP ELISA kit (General species) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Cyclic Adenosine Monophosphate (cAMP). No significant cross-reactivity or interference between Cyclic Adenosine Monophosphate (cAMP) and analogues was observed. |
| Synonyms | c-AMP; 3'-5'-Cyclic Adenosine Monophosphate; Adenosine Cyclophosphate |
| Method | Competitive |
| Detection Range | 246.9-20,000pg/mL |
| Sensitivity | 90.9pg/mL |
| Target | cAMP |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Cyclic Adenosine Monophosphate (cAMP). No significant cross-reactivity or interference between Cyclic Adenosine Monophosphate (cAMP) and analogues was observed. |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately. |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Cold exposure stimulates lipid metabolism, induces inflammatory response in the adipose tissue of mice and promotes the osteogenic differentiation of BMMSCs via the p38 MAPK pathway;Light Emitting Diodes Down‐reCavia (Guinea pig )lates Cathelicidin, Kallikrein, and Toll‐like Receptor 2 Expressions in Keratinocytes and Rosacea‐like Mouse Skin;P2Y12 Promotes Migration of Vascular Smooth Muscle Cells Through Cofilin Dephosphorylation During Atherogenesis.;Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation;Immunomodulatory effect of APS and PSP is mediated by Ca2+-cAMP and TLR4/NF-κB signaling pathway in macrophage.;Ginsenoside Rg5 Inhibits Succinate-Associated Lipolysis in Adipose Tissue and Prevents Muscle Insulin Resistance.;The relation of innate and adaptive immunity with viral-induced acute asthma attacks: Focusing on IP-10 and cathelicidin;Structural re-positioning, molecular modelling, oxidative degradation, and biological screening of linagliptin as adenosine 3 receptor (ADORA3) modulators …;Astragaloside IV Inhibits Adipose Lipolysis and Reduces Hepatic Glucose Production via Akt Dependent PDE3B Expression in HFD-Fed Mice;Dobutamine Up-Regulates Aquaporin 5 Expression in Septic Pulmonary Edema Model In Vitro via cAMP-PKA/CREB Signaling Pathway;Tuberculous meningitis is associated with higher cerebrospinal HIV-1 viral loads compared to other HIV-1-associated meningitides;Developmental competence of buffalo (Bubalus bubalis) denuded oocytes cocultured with cumulus cells: Protective role of cumulus cells;iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine;Calcimimetic R568 improved cardiac remodeling by classic and novel renin-angiotensin system in spontaneously hypertensive rats;Deletion of the serine protease CAP2/Tmprss4 leads to dysregulated renal water handling upon dietary potassium depletion;Cytoplasm lipids can be modulated through hormone-sensitive lipase and are related to mitochondrial function in porcine IVM oocytes;Astragaloside IV relieves gestational diabetes mellitus in genetic mice through reducing hepatic gluconeogenesis;Altered absorptive function in the gall bladder during cholesterol gallstone formation is associated with abnormal NHE3 complex formation;Antihypertensive effects of allicin on spontaneously hypertensive rats via vasorelaxation and hydrogen sulfide mechanisms;DNA microarray analysis of hypothermia-exposed murine lungs for identification of forensic biomarkers;Adrenomedullin 2 attenuates LPS-induced inflammation in microglia cells by receptor-mediated cAMP-PKA pathway;A Green and Blue Monochromatic Light Combination Therapy Reduces Oxidative Stress and Enhances B-Lymphocyte Proliferation through Promoting ¡;Physiological crosstalk between the Mel1a and Mel1c pathways modulates melatonin-mediated, monochromatic light combination-induced bursa B-lymphocyte?¡;The role of adrenergic and muscarinic receptors in stress-induced cardiac injury;Sult2b1 deficiency exacerbates ischemic stroke by promoting pro-inflammatory macrophage polarization in mice; |
| Supplier | Cloud Clone |
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