CAMP ELISA kit (Human)

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CEC419Hu
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Product Name CAMP ELISA kit (Human)
Description This assay has high sensitivity and excellent specificity for detection of Cathelicidin Antimicrobial Peptide (CAMP). No significant cross-reactivity or interference between Cathelicidin Antimicrobial Peptide (CAMP) and analogues was observed.
Synonyms CAP18; FALL39; HSD26; LL37; 18 kDa Cationic Antimicrobial Protein; Antibacterial protein FALL-39; FALL-39 peptide antibiotic
Method Competitive
Detection Range 123.5-10,000ng/mL
Sensitivity 49.2ng/mL
Target CAMP
Specificity This assay has high sensitivity and excellent specificity for detection of Cathelicidin Antimicrobial Peptide (CAMP). No significant cross-reactivity or interference between Cathelicidin Antimicrobial Peptide (CAMP) and analogues was observed.
Reactivity Human
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately.
Uniprot P49913
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References The Assessment of Vitamin D, Antimicrobial Peptides and Procalcitonin in Bronchiectasis;Host immune response to tuberculous meningitis;On birth single dose live attenuated OPV and BCG vaccination induces gut cathelicidin LL37 responses at 6 week of age: A natural experiment;Modulating the Internalization of Bacille Calmette-Guérin by Cathelicidin in Bladder Cancer Cells;Cathelicidin as a link between sarcoidosis and tuberculosis;The association of vitamin D, cathelicidin, and vitamin D binding protein with acute asthma attacks in children;Swiftly Decreasing Cerebrospinal Fluid Cathelicidin Concentration Predicts Improved Outcome in Childhood Bacterial Meningitis.;Short-Term versus Long-Term Culture of A549 Cells for Evaluating the Effects of Lipopolysaccharide on Oxidative Stress, Surfactant Proteins and Cathelicidin LL-37;Hypovitaminosis D and reduced cathelicidin are strongly correlated during the multidrug therapy against leprosy;Compartmentalized Innate Immune Response of Human Fetal Membranes against Escherichia coli Choriodecidual Infection;LL-37 transports immunoreactive cGAMP to activate STING signaling and enhance interferon-mediated host antiviral immunity;
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