| Product Name | CASP3 ELISA kit (Mouse) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Caspase 3 (CASP3). No significant cross-reactivity or interference between Caspase 3 (CASP3) and analogues was observed. |
| Synonyms | CPP32; CPP32B; SCA1; Apoptain; Yama; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 3; SREBP cleavage activity 1 |
| Method | Sandwich |
| Detection Range | 0.625-40ng/mL |
| Sensitivity | 0.229ng/mL |
| Target | CASP3 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Caspase 3 (CASP3). No significant cross-reactivity or interference between Caspase 3 (CASP3) and analogues was observed. |
| Reactivity | Mouse |
| Sample Types | Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P70677 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Role of mitochondria in ciprofloxacin-induced apoptosis in murine sperm cells.;Vitamin D ameliorates hepatic ischemic/reperfusion injury in rats;Inhibition of small HA fragment activity and stimulation of A2A adenosine receptor pathway limit apoptosis and reduce cartilage damage in experimental arthritis;Inhibition of small HA fragment activity and stimulation of A2A;The heat shock protein 90 inhibitor, 17-AAG, attenuates thioacetamide induced liver fibrosis in mice;An integrated proteomics approach shows synaptic plasticity changes in an APP/PS1 Alzheimers mouse model;Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark of Lycium chinense (Cortex Lycii) against gastric ulcer in mice;Chronic low-dose-rate ionising radiation affects the hippocampal phosphoproteome in the ApoE−/− alzheimer mouse model;Protective effects of apigenin and myricetin against cisplatin-induced nephrotoxicity in mice.;Anti-oxidative, anti-secretory and anti-inflammatory activities of the extract from the root bark ofLycium chinense (Cortex Lycii) against gastric ulcer in mice.;Tectorigenin attenuates the MPP+ induced SH‑SY5Y cell damage, indicating a potential beneficial role in Parkinson's disease by oxidative stress inhibition;Pitavastatin inhibits proinflammatory cytokines and provides improvement in terms of its motor function in secondary injuries of the spinal cord mouse models;Potential immunomodulatory and antioxidant effects of walnut vegetable oil against lead‐mediated hepatic damage and their interaction with lipase …;Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice;Mebendazole augments sensitivity to sorafenib by targeting MAPK and BCL-2 signalling in n-nitrosodiethylamine-induced murine hepatocellular carcinoma;Chromon-3-aldehyde derivatives restore mitochondrial function in rat cerebral ischemia; |
| Supplier | Cloud Clone |
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