Cat Acetylcholine receptor subunit alpha (CHRNA1) ELISA Kit

Assay Kits, ELISA, Cat ELISA, Abebio
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Product Name Cat Acetylcholine receptor subunit alpha (CHRNA1) ELISA Kit
Description This assay has high sensitivity and excellent specificity for detection of Cat CHRNA1. No significant cross-reactivity or interference between Cat CHRNA1 and analogues was observed.
Abbreviation CHRNA1
Detection Range 0.156-10 ng/mL
Method Sandwich ELISA
Species Cat
Principle of the Assay This assay employs a two-site sandwich ELISA to quantitate CHRNA1 in samples. An antibody specific for CHRNA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHRNA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRNA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRNA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Sample Types Serum, Plasma, Other biological fluids.
Sensitivity 0.078 ng/mL
Synonyms ACHRA, ACHRD, CHRNA, CMS2A, FCCMS, SCCMS, cholinergic receptor, nicotinic, alpha polypeptide 1 (muscle)|nicotinic acetylcholine receptor alpha subunit|nicotinic cholinergic receptor alpha 1
Uniprot No. P54250

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