| Product Name | CCL25 24-150aa, Human (Denatured) |
|---|---|
| Description | CCL25 also known as C-C motif chemokine 25 isoform 1. CCL25 is potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. It binds to CCR9. Isoform 2 is an antagonist of isoform1 and binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. Recombinant human CCL25-iso1, fused to His-tag at N-terminus, was expressed in E.coli . |
| Synonyms | C-C motif chemokine 25 isoform 1, Ckb15, SCYA25, TECK |
| Host | E. coli |
| Molecular Weight | 33.2kDa (299aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMQGVFE DCCLAYHYPI GWAVLRRAWT YRIQEVSGSC NLPAAIFYLP KRHRKVCGNP KSREVQRAMK LLDARNKVFA KLHHNTQTFQ AGPHAVKKLS SGNSKLSSSK FSNPISSSKR NVSLLISANS GL |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl (pH8.0) containing 10% glycerol |
| Concentration | 0.5mg/ml (determined by Bradford assay) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Watts A.O. et al. (2013) J. Biol. Chem. 288:7169-7181. |
| Background | CCL25 also known as C-C motif chemokine 25 isoform 1. CCL25 is potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. It binds to CCR9. Isoform 2 is an antagonist of isoform1 and binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. Recombinant human CCL25-iso1, fused to His-tag at N-terminus, was expressed in E.coli . |
| Supplier | ARP |
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