| Product Name | CD10 ELISA kit (Human) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Neprilysin (NEP). No significant cross-reactivity or interference between Neprilysin (NEP) and analogues was observed. |
| Synonyms | NEP; MME; CALLA; SFE; Atriopeptidase; Enkephalinase; Skin fibroblast elastase; Common Acute Lymphoblastic Leukemia Antigen; Membrane Metallo-Endopeptidase |
| Method | Sandwich |
| Detection Range | 31.2-2,000pg/mL |
| Sensitivity | 12.3pg/mL |
| Target | CD10 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Neprilysin (CD10). No significant cross-reactivity or interference between Neprilysin (CD10) and analogues was observed. |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cerebrospinal Fluid, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P08473 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Elevated Plasma levels of Neutral Endpeptidase in Hypertension Patients Treated with Angiotensin Converting Enzyme Inhibitors;Soluble neprilysin does not correlate with outcome in heart failure with preserved ejection fraction;A Test in Context: Neprilysin: Function, Inhibition, and Biomarker.;Effects of sacubitril/valsartan on neprilysin targets and the metabolism of natriuretic peptides in chronic heart failure: a mechanistic clinical study;Increased granulocyte membrane neprilysin (CD10) expression is associated with better prognosis in heart failure;Soluble neprilysin, NT-proBNP, and growth differentiation factor-15 as biomarkers for heart failure in dialysis patients (SONGBIRD);P1620 Association of granulocyte neprilysin (CD10) expression with prognosis in heart failure with reduced ejection fraction;Finding a reliable assay for soluble neprilysin;The potential role of serum tau protein (MAPT), neuronal cell adhesion molecule (NrCAM) and neprilysin (NEP) in neurodegenerative disorders development in …; |
| Supplier | Cloud Clone |
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