| Product Name | CD207, 65-328aa, Human (Denatured) |
|---|---|
| Description | CD207 also known as C-type lectin domain family 4 member K. is expressed in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. Additionally it's known by the name C-type lectin domain family 4 member K (CD antigen CD207). It is also expressed in several other dendritic cell types including dermal CD103+ DCs and splenic CD8+ DCs. Langerin is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. It is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. Recombinant human CD207, fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | C-type lectin domain family 4 member K, C-type lectin domain family 4 member K, CLEC4K |
| Host | E. coli |
| Molecular Weight | 33.3kDa (297aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSPRFMGTI SDVKTNVQLL KGRVDNISTL DSEIKKNSDG MEAAGVQIQM VNESLGYVRS QFLKLKTSVE KANAQIQILT RSWEEVSTLN AQIPELKSDL EKASALNTKI RALQGSLENM SKLLKRQNDI LQVVSQGWKY FKGNFYYFSL IPKTWYSAEQ FCVSRNSHLT SVTSESEQEF LYKTAGGLIY WIGLTKAGME GDWSWVDDTP FNKVQSARFW IPGEPNNAGN NEHCGNIKAP SLQAWNDAPC DKTFLFICKR PYVPSEP |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, Liquid, in Phosphate buffered saline (pH7.4) containing 10% glycerol, 1mM DTT |
| Concentration | 1mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Feinberg H., et al. (2013) J. Biol. Chem. 288 (52), 36762-36771 Lauc G., et al. (2013) PLoS Genet. 9 (1), E1003225 |
| Background | CD207 also known as C-type lectin domain family 4 member K. is expressed in Langerhans cells which are immature dendritic cells of the epidermis and mucosa. Additionally it's known by the name C-type lectin domain family 4 member K (CD antigen CD207). It is also expressed in several other dendritic cell types including dermal CD103+ DCs and splenic CD8+ DCs. Langerin is localized in the Birbeck granules, organelles present in the cytoplasm of Langerhans cells and consisting of superimposed and zippered membranes. It is a C-type lectin with mannose binding specificity, and it has been proposed that mannose binding by this protein leads to internalization of antigen into Birbeck granules and providing access to a nonclassical antigen-processing pathway. Recombinant human CD207, fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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