| Product Name | CD27, 21-191aa, Human, hIgG His tag, Baculovirus |
|---|---|
| Description | CD27, as known as CD27 antigen, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycosylation on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD 70 on lymphocytes and dendritic cells. This protein plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. Recombinant human CD27, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Synonyms | CD27 antigen, CD27, S152, LPFS2, T14, TNFRSF7, Tp55, CD 27, CD27 antigen, CD27 molecule, CD27_HUMAN, CD27L receptor, LPFS2, MGC20393, OTTHUMP00000238557, S152, T cell activation antigen CD27, T cell antivation antigen S152, T-cell activation antigen CD27, T14, TNFRSF 7, TNFRSF7, TNFSF7, Tp 55,Tp55, Tumor necrosis factor receptor superfamily member 7 |
| Host | Virus |
| Molecular Weight |
23.7kDa (221aa) 28-40kDa (SDS-PAGE under reducing conditions) |
| Amino Acid Sequence | ADPTPAPKSC PERHYWAQGK LCCQMCEPGT FLVKDCDQHR KAAQCDPCIP GVSFSPDHHT RPHCESCRHC NSGLLVRNCT ITANAECACR NGWQCRDKEC TECDPLPNPS LTARSSQALS PHPQPTHLPY VSEMLEARTA GHMQTLADFR QLPARTLSTH WPPQRSLCSS DFIRLEPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHH HHH |
| Tag | hIgG-His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate Buffered Saline (pH 7.4) containing 40% glycerol |
| Concentration | 0.25mg/ml (determined by Absorbance at 280nm) |
| Purity | > 85% by SDS-PAGE. |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Clemente A., et al. (2013) Clin. Exp. Immunol. 174:97-108. Huang J., et al. (2013) J. Immunol. 190:6250-6258. |
| Background | CD27, as known as CD27 antigen, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycosylation on the cell surface and as a soluble protein in serum. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD 70 on lymphocytes and dendritic cells. This protein plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. Recombinant human CD27, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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