| Product Name | CD5, 25-372aa, Human |
|---|---|
| Description | CD5 is a good immunohistochemical marker for T-cells, although not as sensitive as CD3. About 76% of T-cell neoplasms are reported to express CD5, and it is also found in chronic lymphocytic leukemia, hairy cell leukemia, and mantle cell lymphoma cells. It is commonly lost in cutaneous T-cell lymphoma, and its absence can be used as an indicator of malignancy in this condition. The absence of CD5 in T cell acute lymphoblastic leukemia, while relatively rare, is associated with a poor prognosis. Recombinant human CD5 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | T-cell surface glycoprotein CD5, LEU1, T1 |
| Host | E. coli |
| Molecular Weight | 46.2kDa (411aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSRLSWYDP DFQARLTRSN SKCQGQLEVY LKDGWHMVCS QSWGRSSKQW EDPSQASKVC QRLNCGVPLS LGPFLVTYTP QSSIICYGQL GSFSNCSHSR NDMCHSLGLT CLEPQKTTPP TTRPPPTTTP EPTAPPRLQL VAQSGGQHCA GVVEFYSGSL GGTISYEAQD KTQDLENFLC NNLQCGSFLK HLPETEAGRA QDPGEPREHQ PLPIQWKIQN SSCTSLEHCF RKIKPQKSGR VLALLCSGFQ PKVQSRLVGG SSICEGTVEV RQGAQWAALC DSSSARSSLR WEEVCREQQC GSVNSYRVLD AGDPTSRGLF CPHQKLSQCH ELWERNSYCK KVFVTCQDPN P |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Purity | > 80% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Berland R. et al. (2002) Annu. Rev. Immunol. 20:253–300. Osman N. et al. (1992) Eur. J. Immunol. 22:2995–3000. |
| Background | CD5 is a good immunohistochemical marker for T-cells, although not as sensitive as CD3. About 76% of T-cell neoplasms are reported to express CD5, and it is also found in chronic lymphocytic leukemia, hairy cell leukemia, and mantle cell lymphoma cells. It is commonly lost in cutaneous T-cell lymphoma, and its absence can be used as an indicator of malignancy in this condition. The absence of CD5 in T cell acute lymphoblastic leukemia, while relatively rare, is associated with a poor prognosis. Recombinant human CD5 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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