| Product Name | CDK1, 1-297aa, Human, Baculovirus |
|---|---|
| Description | Cyclin-dependent kinase 1, also known as CDK1, plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. This protein is required in higher cells for entry into S-phase and mitosis. Recombinant human CDK1 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques. |
| Synonyms | Cyclin-dependent kinase 1, CDC2, CDC28A, P34CDC2 |
| Host | Virus |
| Molecular Weight | 36.9 kDa (331aa) confirmed by MALDI-TOF |
| Amino Acid Sequence | MEDYTKIEKI GEGTYGVVYK GRHKTTGQVV AMKKIRLESE EEGVPSTAIR EISLLKELRH PNIVSLQDVL MQDSRLYLIF EFLSMDLKKY LDSIPPGQYM DSSLVKSYLY QILQGIVFCH SRRVLHRDLK PQNLLIDDKG TIKLADFGLA RAFGIPIRVY THEVVTLWYR SPEVLLGSAR YSTPVDIWSI GTIFAELATK KPLFHGDSEI DQLFRIFRAL GTPNNEVWPE VESLQDYKNT FPKWKPGSLA SHVKNLDENG LDLLSKMLIY DPAKRISGKM ALNHPYFNDL DNQIKKMLEH HHHHH |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, Liquid, in Phosphate buffered saline (pH7.4) containing 20% glycerol, 1mM DTT |
| Concentration | 0.25 mg/ml (determined by Bradford assay) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Fourest-Lieuvin A., et al. (2006) Mol. Biol. Cell. 17:1041-1050 Westbrook L., et al. (2007) Cancer Res. 67:11393-11401 |
| Background | Cyclin-dependent kinase 1, also known as CDK1, plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. This protein is required in higher cells for entry into S-phase and mitosis. Recombinant human CDK1 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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