| Product Name | CDK4, 1-303aa, Human |
|---|---|
| Description | Cyclin-dependent kinase 4, also known as CDK4, belongs to the Cyclin-dependent kinases (CDK) family. CDK are the catalytic subunits of the cyclin/cdk complexes, which phosphorylate substrates on threonine/serine residues. CDK4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin upregulates CDK4 activity, whereas binding to the CDK inhibitor p16 downregulates CDK4 activity. Recombinant human CDK4 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Synonyms | Cyclin-dependent kinase 4, CMM3, PSK-J3 |
| Host | E. coli |
| Molecular Weight | 44.4 kDa (400aa), confirmed by MALDI-TOF |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMATSRYE PVAEIGVGAY GTVYKARDPH SGHFVALKSV RVPNGGGGGG GLPISTVREV ALLRRLEAFE HPNVVRLMDV CATSRTDREI KVTLVFEHVD QDLRTYLDKA PPPGLPAETI KDLMRQFLRG LDFLHANCIV HRDLKPENIL VTSGGTVKLA DFGLARIYSY QMALTPVVVT LWYRAPEVLL QSTYATPVDM WSVGCIFAEM FRRKPLFCGN SEADQLGKIF DLIGLPPEDD WPRDVSLPRG AFPPRGPRPV QSVVPEMEES GAQLLLEMLT FNPHKRISAF RALQHSYLHK DEGNPE |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol |
| Concentration | 1 mg/ml (determined by Bradford assay) |
| Purity | > 85% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Serrano M., et al. (1993) Nature.366:704-707. Kato J Y., et al. (1994) Mol Cell Biol. 14:2713-2721. |
| Background | Cyclin-dependent kinase 4, also known as CDK4, belongs to the Cyclin-dependent kinases (CDK) family. CDK are the catalytic subunits of the cyclin/cdk complexes, which phosphorylate substrates on threonine/serine residues. CDK4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin upregulates CDK4 activity, whereas binding to the CDK inhibitor p16 downregulates CDK4 activity. Recombinant human CDK4 protein, fused to His-tag at N-terminus, was expressed in E.coli. |
| Supplier | ARP |
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