| Product Name | CENPH, 136-247aa, Human |
|---|---|
| Description | CENPH, also known as centromere protein H, belongs to the centromere protein H family. This protein is component of the CENPA-NAC (nucleosome-associated) complex that plays a central role in assembly of kinetochore proteins. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Recombinant human CENPH protein, fused to His-tag at N-terminus, was expressed in E.coli and denatured using detergent during a conventional chromatography purification process. |
| Synonyms | Centromere protein H, CENP H, Centromere protein H, ICEN35, Interphase centromere complex protein 35, Kinetochore protein CENP H, NNF1, MIND kinetochore complex component homolog, PMF1 |
| Host | E. coli |
| Molecular Weight | 19.1 kDa (170aa) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MLNKLIMKSQ QESWDLEEKL LDIRKKRLQL KQASESKLLE IQTEKNKQKI DLDSMENSER IKIIRQNLQM EIKITTVIQH VFQNLILGSK VNWAEDPALK EIVLQLEKNV DMM |
| Tag | His-tag |
| Reactivity | Human |
| Applications | SDS-PAGE |
| Form | Liquid, in 20mM Tris-HCl buffer (pH 8.0) , 5% glycerol, 1M Urea. |
| Concentration | 1 mg/ml (determined by Bradford assay ) |
| Purity | > 90% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Saffery R., et al. (2003) Mol Cell. 12(2):509-16. Orthaus S., et al. (2006) Biochem Biophys Res Commun. 15;348(1):36-46. |
| Background | CENPH, also known as centromere protein H, belongs to the centromere protein H family. This protein is component of the CENPA-NAC (nucleosome-associated) complex that plays a central role in assembly of kinetochore proteins. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Recombinant human CENPH protein, fused to His-tag at N-terminus, was expressed in E.coli and denatured using detergent during a conventional chromatography purification process. |
| Supplier | ARP |
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