| Product Name | CFI ELISA kit (Human) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Complement Factor I (CFI). No significant cross-reactivity or interference between Complement Factor I (CFI) and analogues was observed. |
| Synonyms | CF-I; C3b-INA; FI; IF; KAF; Factor I; Konglutinogen-Activating Factor; C3b-Inactivator; C3B/C4B inactivator |
| Method | Sandwich |
| Detection Range | 0.156-10ng/mL |
| Sensitivity | 0.072ng/mL |
| Target | CFI |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Complement Factor I (CFI). No significant cross-reactivity or interference between Complement Factor I (CFI) and analogues was observed. |
| Reactivity | Human |
| Sample Types | Serum, Plasma And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P05156 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | A case report of thrombotic microangiopathy without renal involvement carrying two novel mutations in complement-regulator genes;Atypical hemolytic uremic syndrome: Korean pediatric series;NOVEL ASSAY;Compliment factor i bioassay;A monoclonal antibody targeting amyloid β (Aβ) restores complement factor I bioactivity: Potential implications in age-related macular degeneration and …;The importance of sialic acid, pH and ion concentration on the interaction of uromodulin and complement factor H; |
| Supplier | Cloud Clone |
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