cGMP ELISA kit (General species)

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CEA577Ge
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Product Name cGMP ELISA kit (General species)
Description This assay has high sensitivity and excellent specificity for detection of Cyclic Guanosine Monophosphate (cGMP). No significant cross-reactivity or interference between Cyclic Guanosine Monophosphate (cGMP) and analogues was observed.
Synonyms c-GMP; 3',5'-Cyclic GMP; Cyclic 3',5'-GMP; Guanosine 3',5'-Cyclic Phosphate
Method Competitive
Detection Range 123.5-10,000pg/mL
Sensitivity 49.4pg/mL
Target cGMP
Specificity This assay has high sensitivity and excellent specificity for detection of Cyclic Guanosine Monophosphate (cGMP). No significant cross-reactivity or interference between Cyclic Guanosine Monophosphate (cGMP) and analogues was observed.
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately.
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Cardioprotective effects of baicalein on heart failure via modulation of Ca2;Cardioprotective effects of baicalein on heart failure via modulation of Ca(2+) handling proteins in vivo and in vitro;Influences of Hyriopsis cumingii polysaccharides on mice immunosignaling molecules and T lymphocyte differentiation;Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic rats;ANP/NPRA Inhibits Epithelial-Mesenchymal Transition of Airway by Targeting Smad3 in Asthma;iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine;Atrial natriuretic peptide inhibits epithelial-mesenchymal transition (EMT) of bronchial epithelial cells through cGMP/PKG signaling by targeting Smad3 in a murine …;Effect of cGMP-activated aquaporin 1 on TRPV4 in rats with allodynia induced by chronic compression of the dorsal root ganglion;Effect of tadalafil on penile nitric oxide synthase and corporal smooth muscle in rats under dutasteride treatment;Epigallocatechin gallate mediated sandwich-like coating for mimicking endothelium with sustained therapeutic nitric oxide generation and heparin release;Antihypertensive effects of allicin on spontaneously hypertensive rats via vasorelaxation and hydrogen sulfide mechanisms;The role of adrenergic and muscarinic receptors in stress-induced cardiac injury;A nitric oxide-eluting and REDV peptide-conjugated coating promotes vascular healing;
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