CGRP ELISA kit (Human)

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CEA876Hu
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Product Name CGRP ELISA kit (Human)
Description This assay has high sensitivity and excellent specificity for detection of Calcitonin Gene Related Peptide (CGRP). No significant cross-reactivity or interference between Calcitonin Gene Related Peptide (CGRP) and analogues was observed.
Synonyms CALCB; CGRP2; CALC2; CGRP-II; Calcitonin Gene Related Peptide 2; Calcitonin 2; Calcitonin Related Polypeptide Beta
Method Competitive
Detection Range 6.17-500pg/mL
Sensitivity 2.23pg/mL
Target CGRP
Specificity This assay has high sensitivity and excellent specificity for detection of Calcitonin Gene Related Peptide (CGRP). No significant cross-reactivity or interference between Calcitonin Gene Related Peptide (CGRP) and analogues was observed.
Reactivity Human
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately.
Uniprot P10092
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Alstonia scholaris R. Br. Significantly Inhibits Retinoid-Induced Skin Irritation In Vitro and In Vivo.;Increased levels of CGRP in peripheral blood in women with chronic migraine: A reliable biological marker;CGRP and VIP Levels as Predictors of Efficacy of Onabotulinumtoxin Type A in Chronic Migraine;Prognostic significance of circulating and endothelial progenitor cell markers in type 2 diabetic foot.;Calcitonin gene-and parathyroid hormone-related peptides in normotensive and preeclamptic pregnancies: a nested case–control study;OnabotulinumtoxinA decreases interictal CGRP plasma levels in patients with chronic migraine;Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis;Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients;Transient Receptor Potential Vanilloid Subtype 1 Inhibits Inflammation and Apoptosis via the Release of Calcitonin Gene-Related Peptide in the Heart after Myocardial Infarction.;Probiotic Bifidobacterium longum NCC3001 Reduces Depression Scores and Alters Brain Activity: A Pilot Study in Patients With Irritable Bowel Syndrome;Feasibility of serum CGRP measurement as a biomarker of chronic migraine: a critical reappraisal;Periodontal inflammation is related to increased serum calcitonin gene‐related peptide (CGRP) levels in patients with chronic migraine;Electrical stimulation of the superior sagittal sinus suppresses A-type K currents and increases P/Q-and T-type Ca 2 currents in rat trigeminal ganglion …;Effects of endurance exercise on serum concentration of calcitonin gene-related peptide (CGRP): a potential link between exercise intensity and headache;Serum CGRP, VIP, and PACAP usefulness in migraine: a case–control study in chronic migraine patients in real clinical practice;Mild systemic inflammation enhances response to OnabotulinumtoxinA in chronic migraineurs;Perirenal adipose afferent nerves sustain pathological high blood pressure in rats;
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