CheKine™ Micro Mitochondrial complex I Activity Assay Kit
| Product Name | CheKine™ Micro Mitochondrial complex I Activity Assay Kit |
|---|---|
| Description | Abbkine CheKine™ Micro Mitochondrial Complex I Activity Detection Kit is specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate. |
| Reconstitution | Abbkine CheKine™ Micro Mitochondrial Complex I Activity Detection Kitis specially developed for the detection of complex I activity. The operation is simple and convenient, and the detection is more sensitive and accurate. Complex I can catalyze the dehydrogenation of NADH to NAD+. The oxidation rate of NADH can be determined at 340 nm to calculate the activity of Complex I . |
| Storage | The kit is valid for 6 months. Please refer to the table below Materials supplied and Storage conditions to store all the components. |
| Notes |
• Do not mix the components between different batch numbers and manufacturers; otherwise, the results may be abnormal. • Avoid bubbles while mixing or redissolving components. •Change pipette tips frequently to avoid cross contamination between components. • Ensure that all components and equipment are at the proper temperature before starting the experiment. • In order to guarantee the accuracy of experimental results, need to do a pre-experiment with 1-2 samples. If the absorbance values is too high (above 1.5) or ΔA is greater than 0.4, the samples should be dilute with ReagentⅡand then measured again . Pay attention to multiply by the dilution factor when calculating the result. If ΔA is too small, the sensitivity can be improved by increasing the sample volume added. •The protein concentration of the sample needs to be determined by users. |
| Supplier | Abbkine |
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