CHI3L1 ELISA kit (Human)

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SEB463Hu
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Product Name CHI3L1 ELISA kit (Human)
Description This assay has high sensitivity and excellent specificity for detection of Glycoprotein 39, Cartilage (GP39). No significant cross-reactivity or interference between Glycoprotein 39, Cartilage (GP39) and analogues was observed.
Synonyms GP39; CHI3-L1; YKL40; Chondrex; Chitinase 3-Like 1; Chondrocyte Protein YKL40; 39 kDa synovial protein
Method Sandwich
Detection Range 31.2-2,000pg/mL
Sensitivity 13.5pg/mL
Target CHI3L1
Specificity This assay has high sensitivity and excellent specificity for detection of Chitinase-3-like Protein 1 (CHI3L1). No significant cross-reactivity or interference between Chitinase-3-like Protein 1 (CHI3L1) and analogues was observed.
Reactivity Human
Sample Types Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot P36222
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Serum YKL-40: a potential biomarker for psoriasis or endothelial dysfunction in psoriasis?;YKL-40 is correlated with FEV1 and the asthma control test (ACT) in asthmatic patients: influence of treatment;Assessment of pulsed electromagnetic field therapy with Serum YKL‐40 and ultrasonography in patients with knee osteoarthritis;YKL-40 expression in chronic obstructive pulmonary disease: relation to acute exacerbations and airway remodeling;Compositions and Methods for Diagnosing Lung Cancer;Chronic obstructive pulmonary disease (COPD)--from biomarkers to clinical phenotypes;Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic Patients;High Blood Eosinophil and YKL-40 Levels, as Well as Low CXCL9 Levels, are Associated with Increased Readmission in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease;
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