Chicken Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (MGAT4A) ELISA Kit

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KTE30112
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Product Name Chicken Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (MGAT4A) ELISA Kit
Description Chicken Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (MGAT4A) ELISA Kit has high sensitivity and excellent specificity for detection of Chicken MGAT4A. No significant cross-reactivity or interference between Chicken MGAT4A and analogues was observed. MGAT4A encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A, in addition to the related isoenzyme B, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. This Chicken Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase A (MGAT4A) ELISA Kit employs a two-site sandwich ELISA to quantitate MGAT4A in samples. An antibody specific for MGAT4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMGAT4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MGAT4A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MGAT4A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Synonyms MGAT4A, GNT-IV, GNT-IVA, N-acetylglucosaminyltransferase IVa, OTTHUMP00000161122, OTTHUMP00000203150, UDP-GlcNAc:a-1,3-D-mannoside b-1,4-acetylglucosaminyltransferase IV, UDP-N-acetylglucosamine:alpha1,3-d-mann
Method Sandwich ELISA
Detection Range Please inquire
Sensitivity Please inquire
Reactivity Chicken
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene Id 418692
Storage 2-8°C
Supplier Abbkine

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