Chicken CAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) ELISA Kit

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KTE30061
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Product Name Chicken CAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) ELISA Kit
Description Chicken CAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) ELISA Kit has high sensitivity and excellent specificity for detection of Chicken PRKAR1A. No significant cross-reactivity or interference between Chicken PRKAR1A and analogues was observed. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase A (PKA), which transduces the signal through phosphorylation of different target proteins. Four different regulatory subunits and three catalytic subunits of PKA have been identified in humans. TSE1 is one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Functional mutations in this gene cause Carney complex (CNC), an autosomal dominant multiple neoplasia syndrome. This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. This Chicken CAMP-dependent protein kinase type I-alpha regulatory subunit (PRKAR1A) ELISA Kit employs a two-site sandwich ELISA to quantitate PRKAR1A in samples. An antibody specific for PRKAR1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRKAR1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRKAR1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRKAR1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Synonyms PRKAR1A, CAR, CNC, CNC1, DKFZp779L0468, MGC17251, PKR1, PPNAD1, PRKAR1, TSE1, cAMP-dependent protein kinase regulatory subunit RIalpha, cAMP-dependent protein kinase type I-alpha regulatory chain, cAMP-depende
Method Sandwich ELISA
Detection Range Please inquire
Sensitivity Please inquire
Reactivity Chicken
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene Id 417438
Storage 2-8°C
Supplier Abbkine

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