Chicken Matrilin-3 (MATN3) ELISA Kit

Category: Assay Kits
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KTE30122
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Product Name Chicken Matrilin-3 (MATN3) ELISA Kit
Description Chicken Matrilin-3 (MATN3) ELISA Kit has high sensitivity and excellent specificity for detection of Chicken MATN3. No significant cross-reactivity or interference between Chicken MATN3 and analogues was observed. Matrin 3(MATR3) is localized in the nuclear matrix. It may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. Two transcript variants encoding the same protein have been identified for this gene.The deduced 845-amino acid protein has a calculated molecular mass of approximately 95 kD. Its primary structure consists of 33% charged residues and is generally hydrophilic. Like the lamins, matrin-3 has a positively charged N terminus that contains a large number of amino acids with free hydroxyl groups. A highly acidic domain near the C terminus, in which 32% of the amino acids are acidic, is a characteristic found in other nuclear proteins. This Chicken Matrilin-3 (MATN3) ELISA Kit employs a two-site sandwich ELISA to quantitate MATN3 in samples. An antibody specific for MATN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMATN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MATN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MATN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Synonyms MATN3, DIPOA, EDM5, HOA, OADIP, OS2,
Method Sandwich ELISA
Detection Range Please inquire
Sensitivity Please inquire
Reactivity Chicken
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene Id 395954
Storage 2-8°C
Background Matrin 3(MATR3) is localized in the nuclear matrix. It may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. Two transcript variants encoding the same protein have been identified for this gene.The deduced 845-amino acid protein has a calculated molecular mass of approximately 95 kD. Its primary structure consists of 33% charged residues and is generally hydrophilic. Like the lamins, matrin-3 has a positively charged N terminus that contains a large number of amino acids with free hydroxyl groups. A highly acidic domain near the C terminus, in which 32% of the amino acids are acidic, is a characteristic found in other nuclear proteins.
Supplier Abbkine

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