| Product Name | Chicken Prolyl 3-hydroxylase 2 (LEPREL1) ELISA Kit |
|---|---|
| Description | LEPREL1 belongs to a family of collagen prolyl hydroxylases required for proper collagen biosynthesis, folding, and assembly. The deduced 708-amino acid protein has an N-terminal signal peptide, 4 tetratricopeptide repeats, 4 CxxxC motifs, a central leucine zipper, a prolyl 4-hydroxylase-alpha domain, which contains an ATP/GTP-binding site, and a C-terminal endoplasmic reticulum (ER) retention motif (KDEL). LEPREL1 also has 3 potential N-glycosylation sites and putative glycosaminoglycan attachment sites. It shares 53% and 45% amino acid identity with LEPRE1 and LEPRE2, respectively. Northern blot analysis detected weak expression of a 3.4-kb transcript in most tissues examined, with higher levels in placenta and kidney. A 6.5-kb transcript was expressed abundantly in skeletal muscle and more weakly in heart. This assay has high sensitivity and excellent specificity for detection of Chicken LEPREL1. No significant cross-reactivity or interference between Chicken LEPREL1 and analogues was observed. |
| Synonyms | FLJ10718, MLAT4, P3H2, myxoid liposarcoma associated protein 4|prolyl 3-hydroxylase 2|prolyl 3-hydroxylase 3 |
| Method | Sandwich ELISA |
| Detection Range | 0.312-20 ng/mL |
| Sensitivity | 0.126 ng/mL |
| Reactivity | Chicken |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background | LEPREL1 belongs to a family of collagen prolyl hydroxylases required for proper collagen biosynthesis, folding, and assembly. The deduced 708-amino acid protein has an N-terminal signal peptide, 4 tetratricopeptide repeats, 4 CxxxC motifs, a central leucine zipper, a prolyl 4-hydroxylase-alpha domain, which contains an ATP/GTP-binding site, and a C-terminal endoplasmic reticulum (ER) retention motif (KDEL). LEPREL1 also has 3 potential N-glycosylation sites and putative glycosaminoglycan attachment sites. It shares 53% and 45% amino acid identity with LEPRE1 and LEPRE2, respectively. Northern blot analysis detected weak expression of a 3.4-kb transcript in most tissues examined, with higher levels in placenta and kidney. A 6.5-kb transcript was expressed abundantly in skeletal muscle and more weakly in heart. |
| Supplier | Abebio |
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