| Product Name | Chicken Prosaposin (PSAP) ELISA Kit |
|---|---|
| Description | Chicken Prosaposin (PSAP) ELISA Kit has high sensitivity and excellent specificity for detection of Chicken PSAP. No significant cross-reactivity or interference between Chicken PSAP and analogues was observed. Prosaposin also known as PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Saposin is an acronym for Sphingolipid Activator PrO[S]teINs. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities.Saposins A-D are required for the hydrolysis of certain shingolipids by specific lysosomal hydrolases. This Chicken Prosaposin (PSAP) ELISA Kit employs a two-site sandwich ELISA to quantitate PSAP in samples. An antibody specific for PSAP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPSAP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PSAP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PSAP bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | PSAP, FLJ00245, GLBA, MGC110993, SAP1, sphingolipid activator protein-1 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Chicken |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 395602 |
| Storage | 2-8°C |
| Background | Prosaposin also known as PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Saposin is an acronym for Sphingolipid Activator PrO[S]teINs. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities.Saposins A-D are required for the hydrolysis of certain shingolipids by specific lysosomal hydrolases. |
| Supplier | Abbkine |
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