| Product Name | Chicken Sarcalumenin (SRL) ELISA Kit |
|---|---|
| Description | Chicken Sarcalumenin (SRL) ELISA Kit has high sensitivity and excellent specificity for detection of Chicken SRL. No significant cross-reactivity or interference between Chicken SRL and analogues was observed. Sarcalumenin is a gene which encodes a 160-kD glycoprotein protein involved in calcium signaling.Muscle contraction is triggered by the release of calcium from the sarcoplasmic reticulum, whereas muscle relaxation is achieved by rapid reuptake of calcium from the cytosol into the lumen of the sarcoplasmic reticulum. Sequestration of calcium in the lumen of the sarcoplasmic reticulum is also an essential step in the overall contraction-relaxation cycle of muscle cells. The rabbit sarcoplasmic reticulum contains 2 immunochemically related glycoproteins with apparent molecular masses of 53 and 160 kD.The deduced 160-kD glycoprotein contains an N-terminal signal sequence and a 453-amino acid C-terminal sequence that together form a protein that is identical to the 53-kD glycoprotein. This Chicken Sarcalumenin (SRL) ELISA Kit employs a two-site sandwich ELISA to quantitate SRL in samples. An antibody specific for SRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySRL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SRL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SRL bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | SRL |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Chicken |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 396269 |
| Storage | 2-8°C |
| Background | Sarcalumenin is a gene which encodes a 160-kD glycoprotein protein involved in calcium signaling.Muscle contraction is triggered by the release of calcium from the sarcoplasmic reticulum, whereas muscle relaxation is achieved by rapid reuptake of calcium from the cytosol into the lumen of the sarcoplasmic reticulum. Sequestration of calcium in the lumen of the sarcoplasmic reticulum is also an essential step in the overall contraction-relaxation cycle of muscle cells. The rabbit sarcoplasmic reticulum contains 2 immunochemically related glycoproteins with apparent molecular masses of 53 and 160 kD.The deduced 160-kD glycoprotein contains an N-terminal signal sequence and a 453-amino acid C-terminal sequence that together form a protein that is identical to the 53-kD glycoprotein. |
| Supplier | Abbkine |
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