| Product Name | Chicken Target of Myb protein 1 (TOM1) ELISA Kit |
|---|---|
| Description | Chicken Target of Myb protein 1 (TOM1) ELISA Kit has high sensitivity and excellent specificity for detection of Chicken TOM1. No significant cross-reactivity or interference between Chicken TOM1 and analogues was observed. The N-terminal domain of TOM1 shares sequence similarity with the N-terminal domains of human HGS , human STAM, and yeast VPS27, all of which are proteins associated with vesicular trafficking at the endosome. By searching sequence databases using TOM1 as the query, Seroussi et al. (1999) identified TOM1L1 as another protein with an N-terminal domain similar to that of TOM1. The authors assembled the sequences of a group of human TOM1L1 ESTs into a contiguous cDNA encoding a deduced 476-amino acid protein. TOM1L1 shares 37% sequence identity with TOM1; its C-terminal domain is not similar to that of TOM1. This Chicken Target of Myb protein 1 (TOM1) ELISA Kit employs a two-site sandwich ELISA to quantitate TOM1 in samples. An antibody specific for TOM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOM1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | TOM1, CTA-286B10.4, FLJ33404, target of myb 1, target of myb1 |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Chicken |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 396049 |
| Storage | 2-8°C |
| Background |
The N-terminal domain of TOM1 shares sequence similarity with the N-terminal domains of human HGS , human STAM, and yeast VPS27, all of which are proteins associated with vesicular trafficking at the endosome. By searching sequence databases using TOM1 as the query, Seroussi et al. (1999) identified TOM1L1 as another protein with an N-terminal domain similar to that of TOM1. The authors assembled the sequences of a group of human TOM1L1 ESTs into a contiguous cDNA encoding a deduced 476-amino acid protein. TOM1L1 shares 37% sequence identity with TOM1; its C-terminal domain is not similar to that of TOM1. |
| Supplier | Abbkine |
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