Chicken Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit
| Product Name | Chicken Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit |
|---|---|
| Description | Chicken Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit has high sensitivity and excellent specificity for detection of Chicken MAPKAP1. No significant cross-reactivity or interference between Chicken MAPKAP1 and analogues was observed. Target of rapamycin complex 2 subunit MAPKAP1 is a protein similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. The deduced 522-amino acid protein has a nuclear localization signal, 2 bipartite nuclear localization signals, a peroxisomal targeting signal, and a PEST motif for rapid protein degradation. Comparison of SIN1 with homologs from various species revealed a short, highly conserved region, designated Box1, located within a larger conserved domain, designated CRIM (conserved region in middle). This Chicken Target of rapamycin complex 2 subunit MAPKAP1 (MAPKAP1) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPKAP1 in samples. An antibody specific for MAPKAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPKAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPKAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPKAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | MAPKAP1, RP11-269P11.1, JC310, MGC2745, MIP1, SIN1, SIN1b, SIN1g, MEKK2-interacting protein 1, OTTHUMP00000022141, SAPK-interacting protein 1, ras inhibitor MGC2745, stress-activated map kinase interacting prote |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Chicken |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 395627 |
| Storage | 2-8°C |
| Background |
Target of rapamycin complex 2 subunit MAPKAP1 is a protein similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. The deduced 522-amino acid protein has a nuclear localization signal, 2 bipartite nuclear localization signals, a peroxisomal targeting signal, and a PEST motif for rapid protein degradation. Comparison of SIN1 with homologs from various species revealed a short, highly conserved region, designated Box1, located within a larger conserved domain, designated CRIM (conserved region in middle). |
| Supplier | Abbkine |
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