Ciliary neurotrophic factor, 1-200aa, Rat (Bioactivity Validated)
| Product Name | Ciliary neurotrophic factor, 1-200aa, Rat (Bioactivity Validated) |
|---|---|
| Description | CNTF also known as ciliary neurotrphic factor is a survival factor for various neuronal cell types. CNTF is highly conserved across species and exhibits cross-species bioactivity. CNTF is a 25 kDa polypeptide that was originally isolated as a target-derived survival factor of parasympathetic ciliary ganglion neurons. Binding of CNTF to CNTF receptor triggers heterodimerization of gp130 and LIFR, forming an active trimeric receptor complex and activates the downstream signaling pathway. Recombinant rat CNTF, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Host | E. coli |
| Molecular Weight |
65.5kDa (582aa) 70-100kDa (SDS-PAGE under reducing conditions) |
| Amino Acid Sequence | MGSSHHHHHH SSGLVPRGSH MGSMAFAEQT PLTLHRRDLC SRSIWLARKI RSDLTALMES YVKHQGLNKN INLDSVDGVP VASTDRWSEM TEAERLQENL QAYRTFQGML TKLLEDQRVH FTPTEGDFHQ AIHTLMLQVS AFAYQLEELM VLLEQKIPEN EADGMPATVG DGGLFEKKLW GLKVLQELSQ WTVRSIHDLR VISSHQMGIS ALESHYGAKD KQM |
| Tag | His-tag |
| Reactivity | Rat |
| Applications | SDS-PAGE |
| Form | Liquid, in Phosphate Buffered Saline (pH 7.4) containing 10% glycerol. |
| Concentration | 0.25mg/ml (determined by Absorbance at 280nm) |
| Purity | > 95% by SDS-PAGE |
| Storage | Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -2°C or -7°C. Avoid repeated freezing and thawing cycles. |
| References | Linker R., et al. (2009) Crit Rev Immunol. 29(1):43-68. Stahl N., et al. (1994) J Neurobiol. 25(11):1454-66. |
| Background | CNTF also known as ciliary neurotrphic factor is a survival factor for various neuronal cell types. CNTF is highly conserved across species and exhibits cross-species bioactivity. CNTF is a 25 kDa polypeptide that was originally isolated as a target-derived survival factor of parasympathetic ciliary ganglion neurons. Binding of CNTF to CNTF receptor triggers heterodimerization of gp130 and LIFR, forming an active trimeric receptor complex and activates the downstream signaling pathway. Recombinant rat CNTF, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
| Supplier | ARP |
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