CK18 ELISA kit (Rat)

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SEB231Ra
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Product Name CK18 ELISA kit (Rat)
Description This assay has high sensitivity and excellent specificity for detection of Keratin 18 (KRT18). No significant cross-reactivity or interference between Keratin 18 (KRT18) and analogues was observed.
Synonyms KRT18; CYK18; K18; Keratin 18; Cell proliferation-inducing gene 46 protein; Keratin, type I cytoskeletal 18
Method Sandwich
Detection Range 0.156-10ng/mL
Sensitivity 0.056ng/mL
Target CK18
Specificity This assay has high sensitivity and excellent specificity for detection of Cytokeratin 18 (CK18). No significant cross-reactivity or interference between Cytokeratin 18 (CK18) and analogues was observed.
Reactivity Rat
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot Q5BJY9
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring;Lipidomic‐based investigation into the therapeutic effects of PPC and BBD on rats with NAFLD;Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver …;
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