CKMB ELISA kit (Rat)

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SEA479Ra
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Product Name CKMB ELISA kit (Rat)
Description This assay has high sensitivity and excellent specificity for detection of Creatine Kinase MB Isoenzyme (CKMB). No significant cross-reactivity or interference between Creatine Kinase MB Isoenzyme (CKMB) and analogues was observed.
Synonyms CK2; CK-MB; Creatine Kinase,Muscle/Brain
Method Sandwich
Detection Range 1.56-100ng/mL
Sensitivity 0.67ng/mL
Target CKMB
Specificity This assay has high sensitivity and excellent specificity for detection of Creatine Kinase MB Isoenzyme (CKMB). No significant cross-reactivity or interference between Creatine Kinase MB Isoenzyme (CKMB) and analogues was observed.
Reactivity Rat
Sample Types Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Aldehyde Dehydrogenase-2 Activation during Cardioplegic Arrest Enhances the Cardioprotection against Myocardial Ischemia–Reperfusion Injury;N-Acetylcysteine and Allopurinol Confer Synergy in Attenuating Myocardial Ischemia Injury via Restoring HIF-1α/HO-1 Signaling in Diabetic Rats;Effects of morphine and sufentanil preconditioning against myocardial ischemic-reperfusion injury in rabbits;Effect of levocarnitine on TIMP-1, ICAM-1 expression of rats with coronary heart disease and its myocardial protection effect;In Vivo Cardioprotective Effects and Pharmacokinetic Profile of N-Propyl Caffeamide AgainstIschemia Reperfusion Injury.;Morinda Citrifolia (Noni) and Low Dose Aspirin Prevent Apoptotic Cell Death and Oxidative Stress on Isoproterenol Induced Myocardial Infarction in Rats;Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and;Hydrogen‑rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats;Adiponectin Facilitates Postconditioning Cardioprotection through Both AMPK-Dependent Nuclear and AMPK-Independent Mitochondrial STAT3 Activation;Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injury;Hydrogen sulphide and nitric oxide cooperate in cardioprotection against ischemia/reperfusion injury in isolated rat heart;Biological Safety and Biodistribution of Chitosan Nanoparticles;Mokko Lactone Alleviates Doxorubicin-Induced Cardiotoxicity in Rats via Antioxidant, Anti-Inflammatory, and Antiapoptotic Activities. Nutrients 2022, 14, 733;Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrest;Vitamin E Attenuates Cardiomyopathy Via Alleviation of Autophagic Stress;
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