CLDN3 ELISA kit (Human)

Category: Assay Kits
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SEF293Hu
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Product Name CLDN3 ELISA kit (Human)
Description This assay has high sensitivity and excellent specificity for detection of Claudin 3 (CLDN3). No significant cross-reactivity or interference between Claudin 3 (CLDN3) and analogues was observed.
Synonyms C7orf1; CPE-R2; CPETR2; HRVP1; RVP1; Clostridium Perfringens Enterotoxin Receptor 2; Ventral Prostate.1-Like Protein; CPE-Receptor 2
Method Sandwich
Detection Range 0.312-20ng/mL
Sensitivity 0.112ng/mL
Target CLDN3
Specificity This assay has high sensitivity and excellent specificity for detection of Claudin 3 (CLDN3). No significant cross-reactivity or interference between Claudin 3 (CLDN3) and analogues was observed.
Reactivity Human
Sample Types Serum, Plasma, Tissue Homogenates And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately.
Uniprot O15551
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Gastrointestinal response and endotoxemia during intense exercise in hot and cool environments;Effects of probiotics supplementation on gastrointestinal permeability, inflammation and exercise performance in the heat;Clinical Characteristics Associated With Postoperative Intestinal Epithelial Barrier Dysfunction in Children With Congenital Heart Disease*;Reinforcement of intestinal epithelial barrier by arabinoxylans in overweight and obese subjects: A randomized controlled trial: Arabinoxylans in gut barrier.;Plasma claudin-3 is associated with tumor necrosis factor-alpha-induced intestinal endotoxemia in liver disease;Intestinal Permeability in Children with Celiac Disease after the Administration of Oligofructose-Enriched Inulin into a Gluten-Free Diet—Results of a Randomized …;Markers of Intestinal Damage in Individuals with and without Obesity during a 12-Week Exercise Period.;Dysbiosis and Intestinal Barrier Dysfunction in Pediatric Congenital Heart Disease is Exacerbated Following Cardiopulmonary Bypass;Short-Term Very High Carbohydrate Diet and Gut-Training Have Minor Effects on Gastrointestinal Status and Performance in Highly Trained Endurance Athletes;
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