| Product Name | COL1 ELISA kit (Human) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Collagen Type I (COL1). No significant cross-reactivity or interference between Collagen Type I (COL1) and analogues was observed. |
| Method | Sandwich |
| Detection Range | 0.156-10ng/mL |
| Sensitivity | 0.055ng/mL |
| Target | COL1 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Collagen Type I (COL1). No significant cross-reactivity or interference between Collagen Type I (COL1) and analogues was observed. |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa;Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells;Modification of collagen formation by mesenchymal stem cells isolated from human adipose tissue in culture and after autotransplantation for abdominal hernia plasty;A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches;Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly;Combined effect of strontium and zoledronate on hydroxyapatite structure and bone cell responses;Protective Effects of Norursodeoxycholic Acid Versus Ursodeoxycholic Acid on Thioacetamide-induced Rat Liver Fibrosis;Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media;Carboxymethyl cellulose—hydroxyapatite hybrid hydrogel as a composite material for bone tissue engineering applications;Mast cell chymase in keloid induces profibrotic response via transforming growth factor-β1/Smad activation in keloid fibroblasts;Low-intensity pulsed ultrasound-induced ATP increases bone formation via the P2X7 receptor in osteoblast-like MC3T3-E1 cells;Impact of the Uremic Milieu on the Osteogenic Potential of Mesenchymal Stem Cells;Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses;Multi‐Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co‐Cultured Human Mesenchymal Stem Cells;Incorporation of nanostructured hydroxyapatite and poly(N-isopropylacrylamide) in demineralized bone matrix enhances osteoblast and human mesenchymal stem cell activity;On the Mechanism of Drug Release from Polysaccharide Hydrogels Cross-Linked with Magnetite Nanoparticles by Applying Alternating Magnetic Fields: the Case of DOXO Delivery;Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study;Hybrid Macro-Porous Titanium Ornamented by Degradable 3D Gel/nHA Micro-Scaffolds for Bone Tissue Regeneration;Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study;IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy;Інфаркт міокарда, ускладнений аневризмою лівого шлуночка: клінічні і структурно-функціональні особливості перебігу, діагностичні, терапевтичні та прогностичні аспекти;Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy;Inhibition of hesperidin on epithelial to mesenchymal transition of non-small cell lung cancer cells induced by TGF-β1;Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR;Positive feedback loop of YB-1 interacting with Smad2 promotes liver fibrosis.;Plasma LncRNA-ATB, a Potential Biomarker for Diagnosis of Patients with Coal Workers'Pneumoconiosis: A Case-Control Study.;Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent andmitochondrial pathways in mammalian hepatic stellate cells.;An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells.;Antiresorptive and anti-angiogenetic octacalcium phosphate functionalized with bisphosphonates: An in vitro tri-culture study.;Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells;Demineralized dentin and enamel matrices as suitable substrates for bone regeneration;Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events.;Strontium‐Substituted Hydroxyapatite‐Gelatin Biomimetic Scaffolds Modulate Bone Cell Response;Effect of hyperbaric oxygen therapy (HBO) on implant-associated osteitis in a femur fracture model in mice;BMP-2 and type I collagen preservation in human deciduous teeth after demineralization;Lead exposure inhibits osteoblastic differentiation and inactivates the canonical Wnt signal and recovery by icaritin in MC3T3-E1 subclone 14 cells;Low fibrosis biomarker levels predict cardiac resynchronization therapy response;High amplitude stretching of ATII cells and fibroblasts results in profibrotic effects;Serum sclerostin levels are positively related to bone mineral density in peritoneal dialysis patients: a cross-sectional study;Extracellular matrix deposition by adipose-derived stem cells and fibroblasts: a comparative study;A multifunctional dexamethasone-delivery implant fabricated using atmospheric plasma and its effects on apoptosis, osteogenesis and inflammation;Distinct differences in hypoxic responses between human oral mucosa and skin fibroblasts in a 3D collagen matrix;Predictors of myocardial fibrosis and loss of epicardial adipose tissue volume in the long-term period after myocardial infarction;Ассоциации толщины эпикардиального жира и циркулирующих маркеров фиброза миокарда у пациентов с инфарктом миокарда;Trauma magnitude of the meta-epyphyseal cancellous affects the incidence of deep vein thrombosis. A prospective cohort study on the dynamic of collagen I ¡;Structural Changes and Detection of Liver Fibrosis¨CRelated Protein Levels in Coculture of Alveolar Echinococcosis-Protoscoleces and Human Hepatic Stellate cells;Early diagnosis of myocardial fibrosis in patients with epicardial obesity;Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction;Esomeprazole Alleviates Fibrosis in Systemic Sclerosis by Modulating AhR/Smad2/3 Signaling; |
| Supplier | Cloud Clone |
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