| Product Name | COL1a1 ELISA kit (Human) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 1 (COL1a1). No significant cross-reactivity or interference between Collagen Type I Alpha 1 (COL1a1) and analogues was observed. |
| Synonyms | COL-1A1; COL1-A1; COL1A-1; OI4; Collagen Alpha-1(I)chain |
| Method | Sandwich |
| Detection Range | 31.2-2,000pg/mL |
| Sensitivity | 12.5pg/mL |
| Target | COL1a1 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 1 (COL1a1). No significant cross-reactivity or interference between Collagen Type I Alpha 1 (COL1a1) and analogues was observed. |
| Reactivity | Human |
| Sample Types | Serum, Plasma And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Uniprot | P02452 |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats;Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study ;Porphyromonas gingivalis LPS inhibits osteoblastic differentiation and promotes pro-inflammatory cytokine production in human periodontal ligament stem cells.;Allogeneic human dermal fibroblasts are viable in peripheral blood mononuclear co-culture;Association between Plasma HMGB-1 and Silicosis: A Case-Control Study;Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection;Hesperidin inhibits the epithelial to mesenchymal transition induced by transforming growth factor-β1 in A549 cells through Smad signaling in the cytoplasm;Platinum nanoparticles supported on functionalized hydroxyapatite: Anti-oxidant properties and bone cells response;Antiosteoporotic Nanohydroxyapatite Zoledronate Scaffold Seeded with Bone Marrow Mesenchymal Stromal Cells for Bone Regeneration: A 3D In Vitro Model; |
| Supplier | Cloud Clone |
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