| Product Name | COL2 ELISA kit (Human) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Collagen Type II (COL2). No significant cross-reactivity or interference between Collagen Type II (COL2) and analogues was observed. |
| Method | Sandwich |
| Detection Range | 93.75-6,000pg/mL |
| Sensitivity | 34.43pg/mL |
| Target | COL2 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Collagen Type II (COL2). No significant cross-reactivity or interference between Collagen Type II (COL2) and analogues was observed. |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Tissue Homogenates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Elevated PLGF contributes to small-cell lung cancer brain metastasis;Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells;A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches;Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly;No effects of Hyperosmolar Culture medium on Tissue Regeneration by Human Degenerated Nucleus Pulposus Cells despite Upregulation Extracellular Matrix Genes.;Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media;Co-electrospun gelatin-poly (l-lactic acid) scaffolds: modulation of mechanical properties and chondrocyte response as a function of composition; Electrochemical Immunosensor Detection of Urinary Biomaker for Osteoarthritis Diagnosis ;Highly Porous Gelatin Reinforced 3D Scaffolds for Articular Cartilage Regeneration;The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis;An innovative co-axial system to electrospin in situ crosslinked gelatin nanofibers.;Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders.;Self-complementary adeno-associated virus serotype 6 mediated knockdown of ADAMTS4 induces long-term and effective enhancement of aggrecan in degenerative human nucleus pulposus cells: A new therapeutic approach for intervertebral disc disorders;Platelet-rich concentrate in serum-free medium enhances cartilage-specific extracellular matrix synthesis and reduces chondrocyte hypertrophy of human mesenchymal stromal cells encapsulated in alginate.;A Novel Method to Optimize Autologous Adipose Tissue Recovery with Extracellular Matrix Preservation; |
| Supplier | Cloud Clone |
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