| Product Name | COL3 ELISA kit (Human) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Collagen Type III (COL3). No significant cross-reactivity or interference between Collagen Type III (COL3) and analogues was observed. |
| Method | Sandwich |
| Detection Range | 0.625-40ng/mL |
| Sensitivity | 0.243ng/mL |
| Target | COL3 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Collagen Type III (COL3). No significant cross-reactivity or interference between Collagen Type III (COL3) and analogues was observed. |
| Reactivity | Human |
| Sample Types | Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa;Modification of collagen formation by mesenchymal stem cells isolated from human adipose tissue in culture and after autotransplantation for abdominal hernia plasty;Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly;Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media;Comparative Analysis of the Extracellular Matrix Composition in Proliferating and Involuted Infantile Hemangiomas;IL-17A mediates inflammatory and tissue remodelling events in early human tendinopathy;Інфаркт міокарда, ускладнений аневризмою лівого шлуночка: клінічні і структурно-функціональні особливості перебігу, діагностичні, терапевтичні та прогностичні аспекти;Plasma LncRNA-ATB, a Potential Biomarker for Diagnosis of Patients with Coal Workers'Pneumoconiosis: A Case-Control Study.;Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent andmitochondrial pathways in mammalian hepatic stellate cells.;Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients.;Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells;Distinct differences in hypoxic responses between human oral mucosa and skin fibroblasts in a 3D collagen matrix;Cell sheet technology: Influence of culture conditions on in vitro©\cultivated corneal stromal tissue for regenerative therapies of the ocular surface;Early diagnosis of myocardial fibrosis in patients with epicardial obesity;Manuscript title Microparticles Containing MiR-625-5p In Coronary Artery Reduce Myocardial Fibrosis After Myocardial Infarction; |
| Supplier | Cloud Clone |
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