| Product Name | COL3 ELISA kit (Rat) |
|---|---|
| Description | This assay has high sensitivity and excellent specificity for detection of Collagen Type III (COL3). No significant cross-reactivity or interference between Collagen Type III (COL3) and analogues was observed. |
| Method | Sandwich |
| Detection Range | 0.312-20ng/mL |
| Sensitivity | 0.122ng/mL |
| Target | COL3 |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Collagen Type III (COL3). No significant cross-reactivity or interference between Collagen Type III (COL3) and analogues was observed. |
| Reactivity | Rat |
| Sample Types | Serum, Plasma, Tissue Homogenates And Other Biological Fluids |
| Reconstitution | 1.Prepare all reagents, samples and standards; 2.Add 100uL standard or sample to each well.Incubate 2 hours at 37℃; 3.Aspirate and add 100uL prepared Detection Reagent A.Incubate 1 hour at 37℃; 4.Aspirate and wash 3 times; 5.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 6.Aspirate and wash 5 times; 7.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 8.Add 50uL Stop Solution.Read at 450nm immediately. |
| Storage | 4°C for 1 month/ -20°C for long-term(within shelf life) |
| Notes | Colormetric |
| References | Triptolide inhibits extracellular matrix protein synthesis by suppressing the Smad2 but not the MAPK pathway in TGF-β1-stimulated NRK-49F cells;In vitro tenocyte metabolism in aging and oestrogen deficiency;Effect of nandrolone and/or whey protein on the soleus muscle and testis of adult male albino rats;Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes;Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria;KLF15 is an essential negative regulatory factor for the cardiac remodeling response to pressure overload;The skin remodeling in type 1 diabetes and insulin resistance animal models;The skin remodeling in type 1 diabetes and insulin resistance animal models.;SVVYGLR motif of the thrombin-cleaved N-terminal osteopontin fragment enhances the synthesis of collagen type III in myocardial fibrosis;KLF16 Is an Essential Negative Regulatory Factor for the Cardiac Remodeling Response to Pressure Overload;Crystalline Silica Promotes Rat Fibrocyte Differentiation in Vitro, and Fibrocytes Participate in Silicosis in Vivo;Effects of the (Pro) renin Receptor on Cardiac Remodeling and Function in a Rat Alcoholic Cardiomyopathy Model via the PRR-ERK1/2-NOX4 Pathway; |
| Supplier | Cloud Clone |
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