Cor ELISA kit (General species)

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CEA462Ge
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Product Name Cor ELISA kit (General species)
Description This assay has high sensitivity and excellent specificity for detection of Cortisol (Cor). No significant cross-reactivity or interference between Cortisol (Cor) and analogues was observed.
Synonyms Hydrocortisone
Method Competitive
Detection Range 12.35-1,000ng/mL
Sensitivity 5.15ng/mL
Target Cor
Specificity This assay has high sensitivity and excellent specificity for detection of Cortisol (Cor). No significant cross-reactivity or interference between Cortisol (Cor) and analogues was observed.
Sample Types Serum, Plasma, Urine And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately.
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Use of infrared thermography to assess the influence of high environmental temperature on rabbits;Identification of sociodemographic and clinical factors associated with the levels of human b-defensin-1 and human b-defensin-2 in the human milk of Han Chinese;Daily exposure to summer circadian cycles affects spermatogenesis, but not fertility in an in vivo rabbit model;Effect of high temperatures on breeding rabbit behaviour;Assessment of cortisol as salivary psychological stress marker in relation to temporomandibular disorders among a sample of dental students;Sea buckthorn (Hippophae rhamnoides L.) oil protects against chronic stress-induced inhibitory function of natural killer cells in rats;Effect of thermal stress on physiological, hormonal and haematological parameters in Tharparkar and Karan Fries calves;Relationship between the indexes of insulin resistance and metabolic status in dairy cows during early lactation;Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine …;Endurance exercise causes adverse changes in some hematological and physio-biochemical indices in ponies under high altitude stress condition;Effects of feeding frequency during lactation in primiparous sows on reproduction performance and diurnal rhythm of endocrine profiles at the weaning-to-ovulation …;A study on certain blood biomarkers of pregnant Cholistani cows.;The Use of Infrared Thermography (IRT) as Stress Indicator in Horses Trained for Endurance: A Pilot Study;Saliva cortisol, melatonin levels and circadian rhythm alterations in Chinese primary school children with dyslexia;Assessment of certain biomarkers for predicting survival in response to treatment in dogs naturally infected with canine parvovirus;Effects of dietary nucleotides on growth, survival and metabolic response in whiteleg shrimp, Litopenaeus vannamei against ammonia stress condition;Physiological responses to salinity stress in the Managua Cichlid, Cichlasoma managuense;Anti-stress activity of some plants extracts of the North Caucasus flora;Effect of alfalfa hay and starter feed supplementation on caecal microbiota and fermentation, growth, and health of yak calves;Exploring alterations in hematological and biochemical parameters, enzyme activities and serum cortisol in Besnoitia besnoiti naturally infected dairy cattle;Post©\Cardiac Arrest Hydrocortisone Use Ameliorates Cardiac Mitochondrial Injury in a Male Rat Model of Ventricular Fibrillation Cardiac Arrest;The Protective Role of Alpha-Ketoglutaric Acid on the Growth and Bone Development of Experimentally Induced Perinatal Growth-Retarded Piglets;Stress response after race and endurance training sessions and competitions in Arabian horses;Associations of Chinese social face with cortisol level and glucocorticoid receptor gene;Orexin-A/OX1 Receptor Regulates Autophagy, Mitochondrial Autophagy, and Cortisol Secretion in Human Adrenocortical Cells Through the mTORC1/P70s6k1 …;
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