CORT ELISA kit (Human)

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CEC393Hu
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Product Name CORT ELISA kit (Human)
Description This assay has high sensitivity and excellent specificity for detection of Cortistatin (CORT). No significant cross-reactivity or interference between Cortistatin (CORT) and analogues was observed.
Synonyms CST14; CST17; CST29
Method Competitive
Detection Range 6.17-500ng/mL
Sensitivity 77.24pg/mL
Target CORT
Specificity This assay has high sensitivity and excellent specificity for detection of Cortistatin (CORT). No significant cross-reactivity or interference between Cortistatin (CORT) and analogues was observed.
Reactivity Human
Sample Types Serum, Plasma, Urine And Other Biological Fluids
Reconstitution 1.Prepare all reagents, samples and standards; 2.Add 50uL standard or sample to each well. And then add 50uL prepared Detection Reagent A immediately. Shake and mix.Incubate 1 hour at 37℃; 3.Aspirate and wash 3 times; 4.Add 100uL prepared Detection Reagent B.Incubate 30 minutes at 37℃; 5.Aspirate and wash 5 times; 6.Add 90uL Substrate Solution.Incubate 10-20 minutes at 37℃; 7.Add 50uL Stop Solution.Read at 450 nm immediately.
Uniprot O00230
Storage 4°C for 1 month/ -20°C for long-term(within shelf life)
Notes Colormetric
References Decreased maternal serum cortistatin levels in pregnancies with gestational diabetes mellitus;Microglia Activated by Excess Cortisol Induce HMGB1 Acetylation and Neuroinflammation in the Hippocampal DG Region of Mice Following Cold Exposure;Between pleasure and pain: a pilot study on the biological mechanisms associated with BDSM interactions in dominants and submissives;
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