Bovine 28S ribosomal protein S23, mitochondrial (MRPS23) ELISA Kit

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KTE10269
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Product Name Bovine 28S ribosomal protein S23, mitochondrial (MRPS23) ELISA Kit
Description Bovine 28S ribosomal protein S23, mitochondrial (MRPS23) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MRPS23. No significant cross-reactivity or interference between Bovine MRPS23 and analogues was observed. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS23 encodes a 28S subunit protein. A pseudogene corresponding to MRPS23 is found on chromosome 7p. This Bovine 28S ribosomal protein S23, mitochondrial (MRPS23) ELISA Kit employs a two-site sandwich ELISA to quantitate MRPS23 in samples. An antibody specific for MRPS23 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRPS23 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRPS23 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRPS23 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Synonyms MRPS23, CGI-138, HSPC329, MRP-S23,
Method Sandwich ELISA
Detection Range Please inquire
Sensitivity Please inquire
Reactivity Bovine
Sample Types Cell culture supernatants, Serum, Plasma, Other biological fluids
Gene Id 515228
Storage 2-8°C
Supplier Abbkine

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