| Product Name | Cow Heat shock protein beta-9 (HSPB9) ELISA Kit |
|---|---|
| Description | By searching an EST database for sequences containing the alpha-crystallin domain characteristic of small heat-shock proteins, followed by PCR of genomic DNA, Kappe et al. (2001) cloned HSPB9. The deduced protein contains 159 amino acids. Northern blot analysis of several human tissues detected a 0.8-kb transcript only in testis. In situ hybridization of adult mouse testis showed expression of Hspb9 in spermatogenic cells from late pachytene spermatocyte stage until the elongate spermatid stage. Kappe et al. (2001) determined that the HSPB9 gene is intronless. By genomic sequence analysis, Kappe et al. (2001) mapped the HSPB9 gene to chromosome 17. This assay has high sensitivity and excellent specificity for detection of Bovine HSPB9. No significant cross-reactivity or interference between Bovine HSPB9 and analogues was observed. |
| Synonyms | CT51, FLJ27437, cancer/testis antigen 51|small heat shock protein B9 |
| Method | Sandwich ELISA |
| Detection Range | 0.781-50 ng/mL |
| Sensitivity | 0.29 ng/mL |
| Reactivity | Bovine |
| Sample Types | Serum, Plasma, Other biological fluids. |
| Background |
By searching an EST database for sequences containing the alpha-crystallin domain characteristic of small heat-shock proteins, followed by PCR of genomic DNA, Kappe et al. (2001) cloned HSPB9. The deduced protein contains 159 amino acids. Northern blot analysis of several human tissues detected a 0.8-kb transcript only in testis. In situ hybridization of adult mouse testis showed expression of Hspb9 in spermatogenic cells from late pachytene spermatocyte stage until the elongate spermatid stage. Kappe et al. (2001) determined that the HSPB9 gene is intronless. By genomic sequence analysis, Kappe et al. (2001) mapped the HSPB9 gene to chromosome 17. |
| Supplier | Abebio |
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