| Product Name | Bovine Macrophage-expressed gene 1 protein (MPEG1) ELISA Kit |
|---|---|
| Description | Bovine Macrophage-expressed gene 1 protein (MPEG1) ELISA Kit has high sensitivity and excellent specificity for detection of Bovine MPEG1. No significant cross-reactivity or interference between Bovine MPEG1 and analogues was observed. Macrophage-expressed gene 1 (MPEG1) encodes an evolutionarily conserved protein with a predicted membrane attack complex/perforin domain associated with host defence against invading pathogens. In vertebrates, MPEG1/perforin-2 is an integral membrane protein of macrophages, suspected to be involved in the killing of intracellular bacteria by pore-forming activity. This Bovine Macrophage-expressed gene 1 protein (MPEG1) ELISA Kit employs a two-site sandwich ELISA to quantitate MPEG1 in samples. An antibody specific for MPEG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMPEG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MPEG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MPEG1 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
| Synonyms | MPEG1, MGC132657, MGC138435, MPG1, MPS1 protein, likely ortholog of mouse macrophage expressed gene 1, macrophage expressed gene 1, partial protein |
| Method | Sandwich ELISA |
| Detection Range | Please inquire |
| Sensitivity | Please inquire |
| Reactivity | Bovine |
| Sample Types | Cell culture supernatants, Serum, Plasma, Other biological fluids |
| Gene Id | 539997 |
| Storage | 2-8°C |
| Background | Macrophage-expressed gene 1 (MPEG1) encodes an evolutionarily conserved protein with a predicted membrane attack complex/perforin domain associated with host defence against invading pathogens. In vertebrates, MPEG1/perforin-2 is an integral membrane protein of macrophages, suspected to be involved in the killing of intracellular bacteria by pore-forming activity. |
| Supplier | Abbkine |
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